Typhoid fever is one of the most public health problems in the world with approximately, 17 million cases of typhoid fever worldwide and 600,000 death cases each year. One way to prevent typhoid fever is by vaccination. Nowadays, frequently vaccine for typhoid fever is a vaccine Ty 21a and vi polysaccharide vaccines, but it has the side effect of nausea, fever, and headache after vaccination. So it is still necessary to search other typhoid vaccine with low side effects. The purpose of this study was to obtain information immunogenicity test of recombinant protein Fim-C S. typhimurium and adjuvant alumina in Balb / C mice. The doses used were 20 μg, 40 μg, and 60 μg. Plasma was obtain then analyzed by ELISA, western immunobloting, and dot blot. Immune response of Balb / C mice from KS 1 showed significant differences antibody when dose injection 40μg / 20g BB and KS2 showed significant differences antibody when dose injection 20μg / 20g BB compared with KN. Adjuvant alumina was added to the Fim-C protein inclusion bodies Salmonella typhimurium provides a higher immune response compared to the Fim-C inclusions bodies Salmonella typhimurium protein without the addition of adjuvant alumina. The results of analysis by using western blot and dot blot showed the formation of brown color indicating the interaction between protein Fim-C inclusion bodies Salmonella typhimurium and anti-Fim-C S. typhimurium antibodies. The results of adjuvant alumina and protein Fim-C inclusion bodies of Salmonella typhimurium in Balb / C mice concluded that adjuvant alumina and Fim-C protein inclusion bodies Salmonella typhimurium provide significant increased immune response in Balb / C mice.