Patchoulis (Pogostemon cablin (Blanco) Benth.) is producer plant of essential oil in Indonesia. Now, there is a decrease in productivity of patchouli plant caused Synchytrium pogostemonis attack.This fungi is the cause of the budok disease of patchouli plant and it is an obligate parasite, so it is necessary of biocontrol activity. One of biocontrol activity is used to Actinomycetes. Actinomycetes have some function to pathogen control like a Fusarium, Sclerotium rolfsii and Ralstonia solanaceum and the result will be validation test in the field against Synchytrium pogostemonis. Results showed that 85 Actinomycetes isolates were isolated from 10 plants only 27 isolates have the potential to reduce growth of pathogen from patchouli plant with in vitro. Five selected isolates were tested in the field against Synchytrium pogostemonis. One from five selected isolate have an ability to reduce of budok disease in the field until 80%.