The detection method developed by the UNJ Salmonella team is based on Nucleic Acid-Based methods (genomics). It utilizes a template hybridization process with primers or short nucleotide strands as its complements using the Real-time Polymerase Chain Reaction (Real-Time PCR) technique. The advantages of nucleic-acid-based assays include high specificity and sensitivity results, short time to results process, and better detection quality. The PCR method is still powerful and is widely used in detecting food pathogenic bacteria in the food industry and the pharmaceutical industry.